Guidelines for Sample Submission

We accept protein samples in the following formats:
A. In liquid form
B. In wet or dried polyacrylamide gels. (Wet gels can be submitted in gel-destain or in water).

We require the following information for each sample submission:
1) A gel containing the proteins along with a printed image of the gel indicating what protein spots are to be sequenced. They MUST be numbered sequentially.
2.) Indication of what species the proteins are from and if the proteins have been modified by you.

Click here for guidance on selecting bands to be sequenced!

Gel electrophoresis Guidelines
1) Always use gloves for all phases of sample handling to avoid keratin introduction. Keep gels covered with plastic wrap.
2) Gel thickness should not exceed 1 mm. This is very important! Large amounts of gel inhibits protein identification.
3) Use the Haystead lab protocol for silver staining gels. This method is compatible with mass spectrometry. The method can be found here: Haystead lab silver stain protocol.
4) When loading samples on gels, skip every other lane to allow space for sample excision and to avoid cross contamination. If necessary, multiple (2 or 3) bands can be combined for a single protein.

Getting Your Sample To Us

When your sample is ready bring it to LSRC Rm C119 and ask for Dr. Haystead.

Questions? Contact Dr. Haystead 919-613-8609 or stop by LSRC Rm C119